<- Home <- Arhive <- Vol. 25, Issue 1, March 2017

Rom J Leg Med25(1)92-98(2017)
© Romanian Society of Legal Medicine

Evaluation of the quality of bone powder for successful STR typing of human skeletal remains

I. Z. Pajnič,

Abstract: Aim. We tested the performance of the tissue homogenizer Bead Beater MillMix 20 (Tehtnica - Domel) for grinding the bone samples from skeletal remains for sub sequential extraction and DNA typing of human identification Short Tandem Repeats (STRs).
Methods. We analyzed 60 bone DNA extracts obtained from femurs and tibias. From 13 bones (6 femurs and 7 tibias) analyses were repeated two and from 4 femurs three times to evaluate the repeatability of the extraction method and grinding machine used. DNA was obtained from 0.5 g of demineralizated bone powder and the samples were quantified using the Human Quantifiler or the Investigator Quantiplex kit. STR typing was performed using the NGM or the Investigator ESSplex Plus kit to evaluate the quality of bone powder and tested extraction method.
Results. Of the profiles obtained from 60 DNA extracts, 73% were full, 17% acceptable, 8% quite acceptable and only 2% of the profiles were not suitable for interpretation which represent a 98% success rate. From 17 bones analyses were repeated two or three times and results obtained show high degree of repeatability since very similar results were obtained from almost all of the repetition.
Conclusion. Grinding the bone samples using the MillMix 20 has proven highly successful in recovery of high-quality powder from skeletal remains. We believe that the results obtained will contribute to the possibilities of using automated devices for grinding the bone samples, which would shorten the procedures for obtaining high-quality bone powder from skeletons in forensic laboratories.
Keywords: tissue homogenizer, DNA extraction, autosomal STR typing, bones, skeletal remains, human identification

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